Effect size

One of the widely used effect size is the standardized mean difference between two populations. In practice, because population level information is often not known, this quantity can be estimated from samples collected from the populations. Considering data on a continuous variable for two independent groups, and that the difference between the two groups is of interest, then several different effect sizes could be measured.


Different types of effect sizes
Depending on the nature of a study, the effect size can be measured in the following ways:

effect sizes baseD on Differences between means
One of the widely used effect size is the standardized mean difference between two populations. In practice, because population level information is often not known, this quantity can be estimated from samples collected from the populations. Considering data on a continuous variable for two independent groups, and that the difference between the two groups is of interest, then several different effect sizes could be measured. Cohen's d is the difference between two means, divided by the standard deviation of either group when the variances of the two groups are homogeneous, although in practice, the pooled standard deviation is commonly used. Specifically, Cohen's d is, where x 1 and x 2 are the two sample means, and where n 1 and n 2 are the sample sizes, and s 1 and s 2 are the sample standard deviations of the two groups, respectively. Cohen's d indicates the magnitude of the difference between two means in units of standard deviation. For example, a Cohen's d of 1 indicates that the means of two groups are 1 standard deviation apart. A positive value of Cohen's d indicates that the treatment group has a greater value of whatever was measured than the control group. Negative values indicate that the treatment group has a lower mean than the control group. Hedges

glass's ∆
Glass's ∆ is defined as where s 2 is the standard deviation of the second group, which is often the control group.
There are also other methods to calculate effect size based on differences between means, such as root-mean-square standardized effect and Mahalanobis distance.

effect sizes baseD on "variance explaineD"
In situations in which data are approximately normally distributed and are expected to be evaluated using an ordinary linear regression model, or an ANOVA, the followings are the commonly used effect size measurements.

CoeffiCient of determination r 2
The coefficient of determination R 2 is the square of the Pearson correlation between two continuous variables and measures the proportion of the variance for one variable that is shared with another. Note that, R 2 can be calculated for both simple and multiple-variable linear regression models, and a high R 2 , i.e., a large effect size, often indicates good model fit. However, there are exceptions -sometimes a high R 2 does not means a good fit, and a non-random model residual pattern could tell a different story.

Cohen's f 2
Cohen's f 2 is defined as This is an effect size that can be used in both ANOVA and ordinary linear regression.

effect sizes for associations among categorical variables
There are many types of categorical variables, we focus on situations that the outcome variable is binary, and the exposure variables are categorical.

odds ratio
The odds ratio is a measure of association between an exposure and a binary outcome, and represents the odds that an outcome will occur given a particular exposure category, compared to that in another exposure category. Note that odds ratio can also be calculated for a continuous exposure variable, however, we will not discuss that in this article.

Cohen's h
Cohen's h is defined as where p 1 and p 2 are the proportions of the two samples being compared, and arcsin is the arcsine transformation.

effect size for time-to-event Data
In situations where the outcome of interest is not only whether or not an event has occurred, but also when the event occurred, the commonly used effect size measurement is hazard ratio.
A hazard ratio is the ratio of the hazard rates corresponding to the conditions described by two levels/ categories of an exposure variable, when the exposure variable is categorical.
Note that, while many times effect sizes and treatment effects are used interchangeably, for example, the difference between groups is based on a deliberate intervention; other times, it is more appropriate to use effect size, for example, the difference between males and females.

Effect Size
Yang et al.

effect size anD sample size/power calculation
Statistical power is defined as the probability of finding significant results from a statistical test when an effect actually exists. The factors affecting a power calculation often include sample size, type I error rate (α) and effect size. For example, with a continuous outcome approximately following a normal distribution, the sample size (per group) required for comparing two equal-sized independent groups using a 2-sided two-sample t test is, where z the quantile of a standard normal distribution.
In general, with a pre-specified type I error rate having a small sample size might fail to detect an important effect, while have a large sample size might lead to detect a statistically significant yet clinically insignificant effect. To meaningfully calculate the sample size, a minimal clinically important effect size needs to be specified. This is the smallest difference in outcome between the study groups that is of clinical interest to investigate.

estimation statistics in Data reporting anD presentation
Traditionally, results from a statistical significance test are often used in data reporting and presentation. However, more and more investigators are suggesting that effect size should be included in data reporting and presentation as well.
The primary goal of statistical testing is to obtain a p value, which is the probability of observing results as least as extreme as the observed, when assuming the null hypothesis is true. If the p value of a statistical test is less than a specified value, usually set at 0.05, then we declare statistical significance. On the other hand, effect size evaluates the magnitude of the difference found independent of statistical significance. Therefore, both the significant test and the effect size are complementary and both essential for understanding the differences in a comparison. In fact, effect size has the advantage of quantifying the difference over the use of tests of statistical significance, which could confound with sample size. Many journals have recommendations for manuscript submissions that "avoid relying solely on statistical hypothesis testing, such as p value, which fail to convey important information about effect size." However, specifying only the effect size such as an odds ratio can be just as misleading as specifying only the p value. For rare events, a large odds ratio can still be a very small absolute benefit. Sometimes, a third measure, such as the number to treat to achieve a single benefit, must be added to clarify the situation.

effect size in meta-analysis
A meta-analysis is a statistical analysis that combines results from multiple studies addressing the same question. One important consideration in performing a meta-analysis is to choose an appropriate effect size to be evaluated. For example, the effect size from different studies should be comparable to one another, and should have good technical properties, so that its sampling distribution is known and confidence intervals can be computed.

effect size interpretation
It has been suggested by Cohen that d = 0.2 can be considered a small effect size, 0.5 a medium effect size, and 0.8 a large effect size. This means that, from a statistical consideration, if the difference between the two groups is less than 0.2 standard deviations, then the difference is considered to be small. On the other hand, the interpretation of effect size might be different from a clinical perspective. Depending on the nature of the outcome measured, a 0.2 standard deviation difference might be considered as clinically important for certain outcomes, and a 0.3 standard deviation difference might be considered trivial for other outcomes. There are also differences between clinical and pre-clinical studies. For example, a small but meaningful difference might be important in a clinical study, however, a difference of the same magnitude might not be of interest in a pre-clinical study due to the homogeneity nature of pre-clinical studies.
In summary, effect size is an important quantity in evaluating the strength of the relationship between two variables. Depending on the nature of the outcome variables, there are different types of effect sizes. Choosing the appropriate effect size is not only important in a power/sample size calculation, but also in a meta-analysis. The interpretation of an effect size should take into account both statistical and clinical considerations.